Search results for "Issue 108"

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Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

2016

Relatively quiescent somatic stem cells support life-long cell renewal in most adult tissues. Neural stem cells in the adult mammalian brain are restricted to two specific neurogenic niches: the subgranular zone of the dentate gyrus in the hippocampus and the ventricular-subventricular zone (V-SVZ; also called subependymal zone or SEZ) in the walls of the lateral ventricles. The development of in vivo gene transfer strategies for adult stem cell populations (i.e. those of the mammalian brain) resulting in long-term expression of desired transgenes in the stem cells and their derived progeny is a crucial tool in current biomedical and biotechnological research. Here, a direct in vivo method …

0301 basic medicineEpendymal CellNeurogenesisGeneral Chemical EngineeringGenetic VectorsStem cellsBiologyTransfectionGeneral Biochemistry Genetics and Molecular BiologySubgranular zoneMice03 medical and health sciencesSubependymal zoneNeural Stem CellsEpendymal cellEpendymaLateral VentriclesDevelopmental biologyNichemedicineSubependymal zoneAnimalsNeurogeneticsGeneral Immunology and MicrobiologyLateral ventricleGeneral NeuroscienceLentivirusNeurogenesisGene Transfer TechniquesBrainNeural stem cellCell biology030104 developmental biologymedicine.anatomical_structureVentricular-subventricular zonenervous systemNeural stem cellIssue 108NeurogenèticaStem cellCèl·lules mareDevelopmental biology; Ependymal cell; Issue 108; Lateral ventricle; Lentivirus; Neural stem cell; Neurogenesis; Niche; Subependymal zone; Ventricular-subventricular zone; Animals; Brain; Ependyma; Lateral Ventricles; Lentivirus; Mice; Neural Stem Cells; Transfection; Gene Transfer Techniques; Genetic VectorsDevelopmental biologyNeuroscienceAdult stem cellJournal of Visualized Experiments
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